主要功能：

   ez-gSEAL壓力控制器可輕松實(shí)現(xiàn)膜片鉗。復(fù)雜的膜片鉗實(shí)驗(yàn)變得像點(diǎn)擊幾下按鈕一樣簡(jiǎn)單。單擊**個(gè)按鈕設(shè)置正壓，**個(gè)按鈕設(shè)置負(fù)壓以設(shè)置封接，第三個(gè)按鈕設(shè)置保壓。通過單擊一個(gè)按鈕，您可以應(yīng)用脈沖進(jìn)入細(xì)胞。

  ez-gSEAL 100B壓力控制器主要用于膜片鉗自動(dòng)封接，也可用于其他應(yīng)用。使用ez-gSEAL壓力控制器，細(xì)胞封接就像點(diǎn)擊一個(gè)按鈕一樣簡(jiǎn)單。您可以將它與我們的ezPATCH 100A智能機(jī)械手，PatchMAX 100A自動(dòng)封接膜片鉗系統(tǒng)，ChannelMAX 100A Mini自動(dòng)雙鉗系統(tǒng)或ChannelMAX 100A Twin自動(dòng)四鉗位系統(tǒng)一起使用，用于全自動(dòng)膜片鉗實(shí)驗(yàn)，或?qū)⑵溆米鳘?dú)立產(chǎn)品操作。可以使用控制器來吸食**或研究拉伸通道。軟件控制的壓力控制器配有泵，因此不需要空氣罐。

  與之前的型號(hào)ez-gSEAL 100A壓力控制器，100B有許多改進(jìn)：泵功率更大，使用壽命更長(zhǎng)，設(shè)備內(nèi)的氣流得到改善，性能更佳，并配有壓力表幫助用戶確定用于制作密封件和闖入的壓力。

軟件控制：

ez-gSEAL控制軟件：NBSController控制壓力應(yīng)用并測(cè)量壓力。 常用的壓力可以預(yù)先設(shè)定，因此用戶可以點(diǎn)擊幾個(gè)按鈕進(jìn)行密封和破膜。

新！iPod Touch Control！

ez-gSEAL壓力控制器可由iPod Touch，iPhone或iPAD控制。 iPod Touch通過WiFi連接到服務(wù)器軟件NBSController，因此您可以使用iPod Touch作為遙控器來控制設(shè)備的設(shè)備。

與傳統(tǒng)的密封方法相比，使用ez-gSEAL壓力控制器有許多優(yōu)點(diǎn)：

使用ez-gSEAL壓力控制器，可以在實(shí)驗(yàn)期間設(shè)置非常小的壓力，以使封接持續(xù)很長(zhǎng)時(shí)間。

膜片鉗實(shí)驗(yàn)變得**且可重復(fù)。當(dāng)您使用注射器或口腔式吸入細(xì)胞時(shí)，通常很難控制壓力。使用ez-gSEAL壓力控制器時(shí)，您可以定義吸入壓力和破膜持續(xù)時(shí)間。結(jié)果一致且可靠。在破膜后，膜很難重新封接，但如果發(fā)生這種情況，可以在不接觸微操的情況下施加更多的吸力。

它簡(jiǎn)單而無壓力。無需親自控制壓力，只需點(diǎn)擊幾個(gè)按鈕即可制作封接和破膜,提高工作效率。使用ez-gSEAL，只要記錄密封參數(shù)，就可以輕松達(dá)到正常的密封成功率。成功率高。

自動(dòng)密封算法類似于PatchXpress®自動(dòng)膜片鉗系統(tǒng)中使用的算法。 PatchXpress用戶可以使用它在顯微鏡下測(cè)試其密封參數(shù)，以便更容易地解決問題并降低成本。

來自研究實(shí)驗(yàn)室的數(shù)據(jù)

Figure 1 Recording obtained at the Marine Biological Laboratory using the ez-gSEAL 100A pressure controller. LNV clock neurons in female Drosophila were used for electrophysiological recordings. A 6-7 micrometer diameter pipette filled with protease XIV was first used to digest the tissue above the clock neurons. The pressure setting for applying the protease XIV was 20 - 30 mmHg. When making the seal, 40 mmHg pressure was applied during the time the patch pipette (polished. Resistance ~ 17 MOhms) approached the neuron, and then -20 mmHg suction was applied to make the seal. The breaking-in pressure for this cell was -200 to -250 mmHg for 0.5 s. The holding pressure was _ 1 mmHg to keep the seal stable during the recording. This recording was obtained 3.5 hours after making the seal, and the cell was still healthy.  
Faculties: Dr. Guan Cao, University of Texas; Dr. Michael Nitaback, Yale University.  

Reference: Guan Cao and Michael N. Nitabach (2008) J. Neurosci., 28(25):6493-6501

Figure 2 Suspension of HEK cells expressing ClC-0/C212S chloride channels. When making the seal, 5 mmHg pressure was applied during the time the patch pipette (Resistance ~ 2 MOhms) approached the cell, and then -40 mmHg suction was applied to make the seal. 

Figure 3 Sample AMPAR-and NMDAR-mediated current recorded from VTA DA neuron of p80 and older mouse. When making the seal, 50 mmHg pressure was applied during the time the patch pipette (Resistance ~ 2 MOhms) approached the neuron, and then -20 to -40 mmHg suction was applied to make the seal. The breaking-in pressure for this cell was -150 for 0.5 s.  
Obtained from Dr. Billy Chen, Ernest Gallo Clinic and Research Center, UCSF.

系統(tǒng)技術(shù)指標(biāo)：

壓力范圍：-250 mmHg至250 mmHg

壓力分辨率：+/- 1.5 mmHg

*小脈沖持續(xù)時(shí)間7毫秒

*小脈沖間隔7毫秒

電腦界面USB

電壓：110 VAC至240 VAC

尺寸17“x14”x3.5“機(jī)架或桌面

重量10磅

計(jì)算機(jī)要求：具有2.0 GHz CPU的32位Windows 2000，XP，Vista，Windows8或10系統(tǒng)，1個(gè)USB端口。