別 名
Basement membrane protein 90; BM 90; FBLN; FBLN1;
FIBL 1; FIBL1; Fibulin 1; FBLN1_HUMAN; FIBL-1; Fibulin-1.
交叉反應(yīng)； (predicted: Human, Mouse,
Rat, Chicken, Dog, Pig, Cow, Horse, )
產(chǎn)品應(yīng)用；WB=1:500-2000
ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)）
not yet tested in other
applications.
optimal
dilutions/concentrations should be determined by the end user.
免 疫 原；KLH conjugated synthetic
peptide derived from human Fibulin 1: 501-600/703
亞 型；IgG
純化方法；affinity purified by Protein A
緩 沖 液；0.01M TBS(pH7.4) with 1% BSA,
0.03% Proclin300 and 50% Glycerol.
保存條件；Shipped at 4℃. Store at -20 °C for one year. Avoid
repeated freeze/thaw cycles.
注意事項(xiàng)；This product as supplied is
intended for research use only, not for use in human, therapeutic or diagnostic
applications.
產(chǎn)品介紹：
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Fibulin-1 is an
extracellular matrix protein often associated with fibronectin (FN) in vivo.
Fibulin-1 was found to have pronounced inhibitory effects on the cell
attachment and spreading promoted by FN. Fibulin-1 was also found to inhibit
the motility of a variety of cell types on FN substrata. For example, the
FN-dependent haptotactic motility of breast carcinoma (MDA MB231) cells,
epidermal carcinoma (A431), melanoma (A375 SM), rat pulmonary aortic smooth
muscle cells (PAC1) and Chinese hamster ovary (CHO) cells was inhibited by
the presence of fibulin-1 bound to FN-coated Boyden chamber membranes. Cells
transfected to overproduce fibulin-1 displayed reduced velocity, distance of
movement and persistence time on FN substrata. Similarly, the incorporation
of fibulin-1 into FN-containing type I collagen gels inhibited the invasion
of endocardial cushion mesenchymal cells migrating from cultured embryonic
heart explants. By contrast, incorporation of fibulin-1 into collagen gels
lacking FN had no effect on the migration of endocardial cushion cells.
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