In most cases, colloidal particles possess a positive or negative electrostatic charge. As electrical fields are applied to the particle dispersion, the particles migrate in oppositely charged directions. As particles are irradiated in migration, scattering light causes Doppler shift depending on electrophoresis mobility. NanoPlus software calculates the amount of Doppler shift followed by electrophoretic mobility and zeta potential by combining a heterodyne system and photon correlation method
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