產(chǎn)品簡(jiǎn)介

重組馬白介素-1受體拮抗劑蛋白蛋白與其它公司提供的重組蛋白不同，rEqIL-1RA蛋白產(chǎn)品為采用CFS的無(wú)細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來(lái)的重組蛋白，可表達(dá)出對(duì)細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時(shí)獨(dú)有的全自動(dòng)蛋白純化技術(shù)則簡(jiǎn)便高效，將蛋白純化過(guò)程中對(duì)蛋白的損傷降低到*小程度.重組馬白介素-1受體拮抗劑蛋白蛋白（全長(zhǎng)序列）產(chǎn)品可用于Western Blot驗(yàn)證、抗體制備、蛋白檢測(cè)、ELISA等試驗(yàn)中.

產(chǎn)品詳細(xì)信息

重組馬白介素-1受體拮抗劑蛋白蛋白

Synonyms	IL-1RN, IRAP
Species	Equine
Accession	O18999
GeneID	100034236
Source	重組馬白介素-1受體拮抗劑蛋白蛋白Escherichia coli.
Molecular Weight	Approximately 17.4 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.
Quantity	5μg/20μg/1000μg
AA Sequence	重組馬白介素-1受體拮抗劑蛋白蛋白HPLGKRPCKM QAFRIWDVNQ KTFYMRNNQL VAGYLQESNT KLQEKIDVVP IEPDALFLGL HGRKLCLACV KSGDEIRFQL EAVNITDLSK NKEENKRFTF IRSNSGPTTS FESAACPGWF LCTAQEADRP VSLTNKPKES FMVTKFYLQE DQ
Purity	> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity	Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 3.0 μg/ml, corresponding to a specific activity of > 333 IU/mg in the presence of 50 pg/ml rHuIL-1α.
Physical Appearance	重組馬白介素-1受體拮抗劑蛋白蛋白Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Endotoxin	Less than 1 EU/μg of rEqIL-1RA as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組馬白介素-1受體拮抗劑蛋白蛋白
SDS-PAGE
Reference	1. Dinarello CA. 1994. FASEB J. 8:1314-25. 2. Patterson D, Jones C, Hart I, et al. 1993. Genomics. 15:173-6. 3. Steinkasserer A, Spurr NK, Cox S, et al. 1992. Genomics. 13:654-7. 4. Perrier S, Darakhshan F, Hajduch E. 2006. FEBS Lett. 580:6289-94. 5. Gabay C, Porter B, Fantuzzi G, et al. 1997. J Immunol. 159:5905-13.
Background	IL-1RA was initially called the IL-1 inhibitor which is encoded by the IL1RN gene and it is a member of the interleukin 1 cytokine family. It is secreted by various types of cells including immune cells, epithelial cells, and adipocytes. IL-1RA has functions of inhibiting the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. IL-1RA is also used in the treatment of rheumatoid arthritis, an autoimmune disease in which IL-1 plays a key role. The equus caballus IL-1RA is a single non-glycosylated polypeptide chain containing 152 amino acids and it has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. The protein shows 26 % amino acid homology to IL-1β and 19 % homology to IL-1α. It also shares 78 %~80 % a.a. sequence identity with murine, rat, porcine IL-1RA.